Journal: Oncology Research

Article Title: Unveiling the therapeutic potential: KBU2046 halts triple-negative breast cancer cell migration by constricting TGF-β1 activation in vitro

doi: 10.32604/or.2024.049348

Figure Lengend Snippet: Effects of KBU2046 on integrin family in TNBC. (A and B) Effects of different concentrations of KBU2046 on the expression of integrin αv, integrin α6 in TNBC cells detected by Western blotting. (C and D) Effects of KBU2046 (10 µM) alone or in combination with TGF-β1 (10 ng/mL) on the expression of integrin αv and integrin α6 in TNBC cells detected by Western blotting. Data are presented as means ± SEM of at least three independent experiments. * p < 0.05; * *p < 0.01; ns, no significance. (E and F) The expression and survival probability of integrin αv and in TNBC using the TCGA database.

Article Snippet: Antibodies used included integrin αv (cat. no. 27096-1-AP, Wuhan, China), integrin α6 (cat. no. 27189-1-AP, Wuhan, China), integrin α3 (cat. no. 21992-1-AP, Wuhan, China), and integrin β8 (cat. no. abs105044, Shanghai, China) obtained from Proteintech Group, Inc. ERK1/2 (cat. no. #4695, SD, USA), p-ERK1/2 (Thr202/Tyr204) (#9101, SD, USA), Raf1 (#9422, SD, USA), and p-Raf1 (Ser338) (#9427, SD, USA) were sourced from Cell Signaling Technology, Inc.

Techniques: Expressing, Western Blot

Journal: Oncology Research

Article Title: Unveiling the therapeutic potential: KBU2046 halts triple-negative breast cancer cell migration by constricting TGF-β1 activation in vitro

doi: 10.32604/or.2024.049348

Figure Lengend Snippet: Effects of KBU2046 on integrin α3 and integrin β8 in TNBC. (A and B) Effects of different concentrations of KBU2046 on the expression of integrin α3, integrin β8 in TNBC cells detected by Western blotting. ns, no significance.

Article Snippet: Antibodies used included integrin αv (cat. no. 27096-1-AP, Wuhan, China), integrin α6 (cat. no. 27189-1-AP, Wuhan, China), integrin α3 (cat. no. 21992-1-AP, Wuhan, China), and integrin β8 (cat. no. abs105044, Shanghai, China) obtained from Proteintech Group, Inc. ERK1/2 (cat. no. #4695, SD, USA), p-ERK1/2 (Thr202/Tyr204) (#9101, SD, USA), Raf1 (#9422, SD, USA), and p-Raf1 (Ser338) (#9427, SD, USA) were sourced from Cell Signaling Technology, Inc.

Techniques: Expressing, Western Blot

Journal: Journal of thrombosis and haemostasis : JTH

Article Title: Mechanisms underlying platelet function defect in a pedigree with familial platelet disorder with a predisposition to acute myelogenous leukemia: potential role for candidate RUNX1 targets.

doi: 10.1111/jth.12550

Figure Lengend Snippet: Fig. 5. Outside-in signaling events in platelets from patients with familial platelet disorder with a predisposition to acute myelogenous leuke- mia. (A) Platelets were plated on fibrinogen-coated coverslips, and actin fibers were labeled with fluorescein isothiocyanate–phalloidin. As com- pared with controls (n = 3), patients (n = 3) show a trend for a higher percentage of platelets remaining round or showing only filopodia, and a decrease in lamellipodia extension. Data are expressed as mean standard error of the mean, P > 0.05. (B) Representative image showing the morphology of platelets adherent to fibrinogen in a control sample (left panel) and a patient (right panel) sample. Scale bar: 5 lm (C) Wes- tern blot analysis of b3 integrin phosphorylation is shown in (i). Washed platelets were layered over fibrinogen-coated plates for 60 min. After removal of non-adherent cells, the reaction was stopped with lysis buffer, and lysates were subjected to SDS-PAGE and probed with anti-phos- pho -b3 integrin (Tyr773) antibody. Protein loading was assessed with anti-b3 integrin antibody. Control platelets show enhanced b3 integrin phosphorylation upon adherence to immobilized fibrinogen as compared with non-adherent platelets, whereas the response is blunted in patient platelets. Densitometric analysis of band intensity is shown in (ii).

Article Snippet: After SDS-PAGE, membranes were incubated with rabbit polyclonal anti-phospho-b3 integrin (Tyr773) antibody (Abcam, Cambridge, UK) followed by horseradish peroxidase (HRP)-conjugated anti-rabbit antibody; enhanced chemiluminescence (ECL) detection was then performed.

Techniques: Labeling, Control, Phospho-proteomics, Lysis, SDS Page